Molecular Probes of Spermatozoan Structures

The Fine Structure of Haematolœchus Spermatozoan Tail

For a n u m b e r of year~ investigators generally agreed that electron optical studies had established a basic pattern for the axial fiber bundle of sperm tails. This consisted essentially of two single ilia-ments centered within a ring made up of nine doublet filaments. It was further established that this structure possessed bilateral symmetry, All of the electron micrographs are from sperma...

Molecular and Cellular Probes

Real-time PCR was used to analyze archived blood from non-human primates (NHP) and fluid samples originating from a well-controlled Q fever vaccine efficacy trial. The PCR targets were the IS1111 element and the com1 gene of Coxiella burnetii. Data from that previous study were used to evaluate real-time PCR as an alternative to the use of sero-conversion by mouse bioassay for both quantificati...

Mycology with molecular probes.

Sensitivity, specificity, simplicity, speed, and economy fairly well describe the desirable attributes of any clinical diagnostic test. In the mycology laboratory, these conditions unevenly apply to detection and identification of various organisms. They are adequate for the routinely encountered pathogens, but for the opportunistic and emerging pathogens the situation is not optimal. This occu...

Development of RNA aptamers as molecular probes for HER2+ breast cancer study using cell-SELEX

Objective(s): Development of molecules that specifically recognize cancer cells is one of the major areas in cancer research. Human epidermal growth factor receptor 2 (HER2) is specifically expressed on the surface of breast cancer cells. HER2 is associated with an aggressive phenotype and poor prognosis. In this study we aimed to isolate RNA aptamers that specifically bind to HER2 overexpressi...

Spectroscopic probes of molecular recognition.